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ipsc line bxs0114  (ATCC)


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    ATCC ipsc line bxs0114
    Ipsc Line Bxs0114, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ipsc+line+bxs0114/ATCC-BXS0114+Human+%5BAfrican+American+Female%5D+Induced+Pluripotent+Stem+(IPS)+Cells/pm38838223-182-21-31
    Average 93 stars, based on 17 article reviews
    ipsc line bxs0114 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

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    Article Title: Interferon-γ induces combined pyroptotic angiopathy and APOL1 expression in human kidney disease.
    Article Snippet: 1016SevA iPSCs This cell line was derived from fibroblasts from amale non-African ancestry donor at the Harvard StemCell Institute with the identifier RRID:CVCL_UK18. .. This embryonic stem cell line was generated at the University of Michigan and submitted to the NIH with registration no. 0278. iPSC line BXS0114 This cell line is commercially available from ATCC under the identifier ACS-1028 and was derived from the bone marrow of a 31 year old African American Female. iPSC line BYS0110 This cell line is commercially available from ATCC under the identifier ACS-1024 and was derived from the bone marrow of a 33 year old African American male. .. This embryonic stem cell line was generated at the University of Michigan and submitted to the NIH with registration no. 0278. iPSC line BXS0114 This cell line is commercially available from ATCC under the identifier ACS-1028 and was derived from the bone marrow of a 31 year old African American Female. iPSC line BYS0110 This cell line is commercially available from ATCC under the identifier ACS-1024 and was derived from the bone marrow of a 33 year old African American male.

    Derivative Assay:

    Article Title: Interferon-γ induces combined pyroptotic angiopathy and APOL1 expression in human kidney disease.
    Article Snippet: 1016SevA iPSCs This cell line was derived from fibroblasts from amale non-African ancestry donor at the Harvard StemCell Institute with the identifier RRID:CVCL_UK18. .. This embryonic stem cell line was generated at the University of Michigan and submitted to the NIH with registration no. 0278. iPSC line BXS0114 This cell line is commercially available from ATCC under the identifier ACS-1028 and was derived from the bone marrow of a 31 year old African American Female. iPSC line BYS0110 This cell line is commercially available from ATCC under the identifier ACS-1024 and was derived from the bone marrow of a 33 year old African American male. .. This embryonic stem cell line was generated at the University of Michigan and submitted to the NIH with registration no. 0278. iPSC line BXS0114 This cell line is commercially available from ATCC under the identifier ACS-1028 and was derived from the bone marrow of a 31 year old African American Female. iPSC line BYS0110 This cell line is commercially available from ATCC under the identifier ACS-1024 and was derived from the bone marrow of a 33 year old African American male.



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    Mitochondria accumulate over serial passages in <t>ACS1028-ECs</t> without a compensatory increase in recycling. ( A ) Top panel: MitoTracker staining of ACS1028 cells on each day of differentiation to ECs demonstrated that mitochondrial mass and morphology change over the course of differentiation. Middle panel: MitoTracker staining of each passage of ACS1028-ECs showed mitochondrial accumulation in later passages. Bottom panel: Quantification of average MitoTracker signal per cell by fluorescence microscopy revealed a significant and greater than 20-fold increase in mitochondria in iPSC-ECs ( P < .05). ( B ) LC3-II expression remained stable throughout serial passages of Y6-ECs. ( C-D ) Representative TEM images of Y6-ECs from each passage showing a stable number of autolysosomes over serial passages. ( E ) Quantification of autolysosomes containing mitochondrial structures demonstrated a decrease in mitophagy in Y6-ECs compared to during differentiation ( P < .05).
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    Mitochondria accumulate over serial passages in <t>ACS1028-ECs</t> without a compensatory increase in recycling. ( A ) Top panel: MitoTracker staining of ACS1028 cells on each day of differentiation to ECs demonstrated that mitochondrial mass and morphology change over the course of differentiation. Middle panel: MitoTracker staining of each passage of ACS1028-ECs showed mitochondrial accumulation in later passages. Bottom panel: Quantification of average MitoTracker signal per cell by fluorescence microscopy revealed a significant and greater than 20-fold increase in mitochondria in iPSC-ECs ( P < .05). ( B ) LC3-II expression remained stable throughout serial passages of Y6-ECs. ( C-D ) Representative TEM images of Y6-ECs from each passage showing a stable number of autolysosomes over serial passages. ( E ) Quantification of autolysosomes containing mitochondrial structures demonstrated a decrease in mitophagy in Y6-ECs compared to during differentiation ( P < .05).
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    Mitochondria accumulate over serial passages in <t>ACS1028-ECs</t> without a compensatory increase in recycling. ( A ) Top panel: MitoTracker staining of ACS1028 cells on each day of differentiation to ECs demonstrated that mitochondrial mass and morphology change over the course of differentiation. Middle panel: MitoTracker staining of each passage of ACS1028-ECs showed mitochondrial accumulation in later passages. Bottom panel: Quantification of average MitoTracker signal per cell by fluorescence microscopy revealed a significant and greater than 20-fold increase in mitochondria in iPSC-ECs ( P < .05). ( B ) LC3-II expression remained stable throughout serial passages of Y6-ECs. ( C-D ) Representative TEM images of Y6-ECs from each passage showing a stable number of autolysosomes over serial passages. ( E ) Quantification of autolysosomes containing mitochondrial structures demonstrated a decrease in mitophagy in Y6-ECs compared to during differentiation ( P < .05).
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    Image Search Results


    Mitochondria accumulate over serial passages in ACS1028-ECs without a compensatory increase in recycling. ( A ) Top panel: MitoTracker staining of ACS1028 cells on each day of differentiation to ECs demonstrated that mitochondrial mass and morphology change over the course of differentiation. Middle panel: MitoTracker staining of each passage of ACS1028-ECs showed mitochondrial accumulation in later passages. Bottom panel: Quantification of average MitoTracker signal per cell by fluorescence microscopy revealed a significant and greater than 20-fold increase in mitochondria in iPSC-ECs ( P < .05). ( B ) LC3-II expression remained stable throughout serial passages of Y6-ECs. ( C-D ) Representative TEM images of Y6-ECs from each passage showing a stable number of autolysosomes over serial passages. ( E ) Quantification of autolysosomes containing mitochondrial structures demonstrated a decrease in mitophagy in Y6-ECs compared to during differentiation ( P < .05).

    Journal: Stem Cells Translational Medicine

    Article Title: Autophagy Enhances Longevity of Induced Pluripotent Stem Cell-Derived Endothelium via mTOR-Independent ULK1 Kinase

    doi: 10.1093/stcltm/szac069

    Figure Lengend Snippet: Mitochondria accumulate over serial passages in ACS1028-ECs without a compensatory increase in recycling. ( A ) Top panel: MitoTracker staining of ACS1028 cells on each day of differentiation to ECs demonstrated that mitochondrial mass and morphology change over the course of differentiation. Middle panel: MitoTracker staining of each passage of ACS1028-ECs showed mitochondrial accumulation in later passages. Bottom panel: Quantification of average MitoTracker signal per cell by fluorescence microscopy revealed a significant and greater than 20-fold increase in mitochondria in iPSC-ECs ( P < .05). ( B ) LC3-II expression remained stable throughout serial passages of Y6-ECs. ( C-D ) Representative TEM images of Y6-ECs from each passage showing a stable number of autolysosomes over serial passages. ( E ) Quantification of autolysosomes containing mitochondrial structures demonstrated a decrease in mitophagy in Y6-ECs compared to during differentiation ( P < .05).

    Article Snippet: The iPSC line ACS1028 was obtained from ATCC (ATCC-BXS0114) and were cultured on vitronectin-coated plates (Thermo Fisher A31804) with E8 flex media (Gibco A2858501).

    Techniques: Staining, Fluorescence, Microscopy, Expressing

    Hypoxia reduced MitoTracker staining per ACS1028-EC cell at each passage but still resulted in loss of proliferation over serial passages. ( A ) Schematic diagram of hypoxia experiment. ( B ) 1.5% oxygen reduced MitoTracker red and green staining per ACS1028-EC cell compared to normoxic conditions at early passages but cells ultimately accumulated mitochondria in later passages. ( C ) ACS1028-EC proliferation was improved under hypoxic conditions but still declined over serial passages.

    Journal: Stem Cells Translational Medicine

    Article Title: Autophagy Enhances Longevity of Induced Pluripotent Stem Cell-Derived Endothelium via mTOR-Independent ULK1 Kinase

    doi: 10.1093/stcltm/szac069

    Figure Lengend Snippet: Hypoxia reduced MitoTracker staining per ACS1028-EC cell at each passage but still resulted in loss of proliferation over serial passages. ( A ) Schematic diagram of hypoxia experiment. ( B ) 1.5% oxygen reduced MitoTracker red and green staining per ACS1028-EC cell compared to normoxic conditions at early passages but cells ultimately accumulated mitochondria in later passages. ( C ) ACS1028-EC proliferation was improved under hypoxic conditions but still declined over serial passages.

    Article Snippet: The iPSC line ACS1028 was obtained from ATCC (ATCC-BXS0114) and were cultured on vitronectin-coated plates (Thermo Fisher A31804) with E8 flex media (Gibco A2858501).

    Techniques: Staining

    Plant derivative Rg2 improved proliferation of ACS1028-ECs over serial passages. ( A ) EdU labeling of ACS1028-ECs treated with Rg2, rapamycin or DMSO as a vehicle control demonstrated improved proliferation with Rg2 treatment. ( B ) Quantification of EdU labeled cells demonstrated a statistically significant improvement in proliferation with Rg2 treatment compared to treatment with rapamycin, ML246, or mitophagy inducers Antimycin A or oligomycin. ( C ) The benefit to proliferation conferred by Rg2 was sustained through passage 3.

    Journal: Stem Cells Translational Medicine

    Article Title: Autophagy Enhances Longevity of Induced Pluripotent Stem Cell-Derived Endothelium via mTOR-Independent ULK1 Kinase

    doi: 10.1093/stcltm/szac069

    Figure Lengend Snippet: Plant derivative Rg2 improved proliferation of ACS1028-ECs over serial passages. ( A ) EdU labeling of ACS1028-ECs treated with Rg2, rapamycin or DMSO as a vehicle control demonstrated improved proliferation with Rg2 treatment. ( B ) Quantification of EdU labeled cells demonstrated a statistically significant improvement in proliferation with Rg2 treatment compared to treatment with rapamycin, ML246, or mitophagy inducers Antimycin A or oligomycin. ( C ) The benefit to proliferation conferred by Rg2 was sustained through passage 3.

    Article Snippet: The iPSC line ACS1028 was obtained from ATCC (ATCC-BXS0114) and were cultured on vitronectin-coated plates (Thermo Fisher A31804) with E8 flex media (Gibco A2858501).

    Techniques: Labeling, Control

    Ulk1 kinase inhibitor SBI-0206965 blocked the survival benefit associated with increased LC3-II that was conferred by Rg2. ( A ) Manual counting of cells in light microscopy demonstrated a statistically significant reduction in cell survival in a dose-dependent manner when cells were co-treated with SBI-0206965 in the presence of Rg2. ( B-C ) Western blotting of ACS1028-EC lysate from serial passages treated with DMSO as a control, or with Rg2, or with Rg2 and various concentrations of SBI-0206965 demonstrated a significant increase in LC3-II expression with Rg2 treatment at passage 3 that is attenuated by co-treatment with SBI-0206965.

    Journal: Stem Cells Translational Medicine

    Article Title: Autophagy Enhances Longevity of Induced Pluripotent Stem Cell-Derived Endothelium via mTOR-Independent ULK1 Kinase

    doi: 10.1093/stcltm/szac069

    Figure Lengend Snippet: Ulk1 kinase inhibitor SBI-0206965 blocked the survival benefit associated with increased LC3-II that was conferred by Rg2. ( A ) Manual counting of cells in light microscopy demonstrated a statistically significant reduction in cell survival in a dose-dependent manner when cells were co-treated with SBI-0206965 in the presence of Rg2. ( B-C ) Western blotting of ACS1028-EC lysate from serial passages treated with DMSO as a control, or with Rg2, or with Rg2 and various concentrations of SBI-0206965 demonstrated a significant increase in LC3-II expression with Rg2 treatment at passage 3 that is attenuated by co-treatment with SBI-0206965.

    Article Snippet: The iPSC line ACS1028 was obtained from ATCC (ATCC-BXS0114) and were cultured on vitronectin-coated plates (Thermo Fisher A31804) with E8 flex media (Gibco A2858501).

    Techniques: Light Microscopy, Western Blot, Control, Expressing